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Shotgun concatenation of synthetic genes: construction of concatemeric human calcitonin genes

✍ Scribed by Ivan G. Ivanov

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 386 KB
Volume: 189
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(90)90110-u

No coin nor oath required. For personal study only.

✦ Synopsis

A new fast and reliable approach for construction of concatemeric synthetic genes is described. The method consists of shotgun polymerization of three truncated monomeric gene units using a specific linker, followed by cloning of the recombinant clones and screening them for the presence of concatemeric genes of defined length. The method is illustrated by constructing a series of tandemly repeated human calcitonin (hCT) genes.

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