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Short-time phosphorylation of plasma membrane proteins by endogenous kinases

✍ Scribed by Thomas Joos; F.Alfred Anderer

Book ID
118312491
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
395 KB
Volume
84
Category
Article
ISSN
0006-291X

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Incubation of plasma membranes isolated from bovine aorta with either 0.5 mM CaCl2 or with a phorbol ester (1 microM phorbol 12,13-dibutyrate) and phosphatidylserine in an EGTA-containing buffer resulted in the phosphorylation of 10 proteins (Mr of 158, 105, 75, 62, 44, 39, 33, 22, 15 and 9 kDa), pr