Short-term cryopreservtion of human breast carcinoma cells for flow cytometry
β Scribed by Kenneth R. Stone; R. Bruce Craig; James O. Palmer; Saul E. Rivkin; Robert W. McDivitt
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 467 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
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## Abstract Various growth media and procedures for tissue disaggregation and culturing were tested with regard to cell attachment, the type of cells to grow out, and the emergence of cytogenetically abnormal clones in cultures of 20 primary breast carcinomas. Clonal chromosome abnormalities were d
## Background: Between january 11, 1991 and january 8, 1992, 104 patients with previously untreated, invasive, primitive breast carcinoma were admitted to the authors' hospital. ## Methods: For each patient, flow cytometry dna analyses on frozen samples and on immunohistochemical staining were pe