Short strong hydrogen bonds in proteins: a case study of rhamnogalacturonan acetylesterase

✍ Scribed by Langkilde, Annette ;Kristensen, Søren M. ;Lo Leggio, Leila ;Mølgaard, Anne ;Jensen, Jan H. ;Houk, Andrew R. ;Navarro Poulsen, Jens-Christian ;Kauppinen, Sakari ;Larsen, Sine

• Book ID: 104478408
• Publisher: International Union of Crystallography
• Year: 2008
• Tongue: English
• Weight: 987 KB
• Volume: 64
• Category: Article
• ISSN: 0907-4449
• DOI: 10.1107/s0907444908017083

✦ Synopsis

The short hydrogen bonds in rhamnogalacturonan acetylesterase have been investigated by structure determination of an active-site mutant, ^1^H NMR spectra and computational methods. Comparisons are made to database statistics. A very short carboxylic acid carboxylate hydrogen bond, buried in the protein, could explain the low-field (18 p.p.m.) ^1^H NMR signal.