Short communication: Purification and properties of a glucose-forming amylase ofLactobacillus brevis

✍ Scribed by M.O. Ilori; O.O. Amund; O. Omidiji

• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 176 KB
• Volume: 11
• Category: Article
• ISSN: 1573-0972
• DOI: 10.1007/bf00286381

✦ Synopsis

An extracellular glucose-forming amylase was produced by Lactobacillus brevis isolated from 'Kagasok tea'. The enzyme was purified 70-fold and had optimal activity at 55°C and pH 6.5. Its K m value for starch was 0.27 mg ml(-1) and its M r was approx. 75,900 Da. The activity of the enzyme was enhanced by Ca(2+), Mg(2+), Na(+) or K(+) and inhibited by EDTA, KCN, citric acid and L-cysteine.