Short communication: Production ofl-glutamate oxidase byStreptomycessp. N1 in submerged fermentation

✍ Scribed by Q.-S. Li; J.-J. Zhong

• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 192 KB
• Volume: 12
• Category: Article
• ISSN: 1573-0972
• DOI: 10.1007/bf00327732

✦ Synopsis

In submerged fermentation of Streptomyces sp. N1 in a shake flask, glucose (3% w/v) and (NH4)2SO4 (0.6% w/v) were found to be suitable for extracellular L-glutamate oxidase (GluOx) (EC.1.4.3.11) production. GluOx production was higher with the addition of further KCl or MgCl2 to the medium within the range of 0 to 0.12% (w/v). The effect of inoculum type, that is, spore inoculation or mycelium inoculation on GluOx biosynthesis was also investigated, and the maximum GluOx production obtained was 2.7 U/ml after 33h fermentation with mycelium inoculation. The results demonstrated a much higher GluOx production and productivity compared with those reported previously.