✦ LIBER ✦
Short-Column Gradient Elution High-Performance Liquid Chromatography for the Rapid Determination of 7-Ethoxycoumarin Metabolites in Liver-Slice Incubates
✍ Scribed by D.L. Hiller; R.O. Cole
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 259 KB
- Volume
- 227
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
An improved HPLC method for the analysis of 7-ethoxycoumarin and its metabolites in liver-slice incubation media is presented. The assay is based on short-column gradient elution which permits baseline resolution of the parent drug and three metabolites with a fourfold reduction in analysis time relative to existing HPLC methods. Total assay run time is (10 \mathrm{~min}). Data establishing method performance are presented which show intra- and interassay precision and accuracy to be within 13 and (7 %), respectively, for separately prepared quality control samples. c 1995 Academic Press, Inc.