Short chain phospholipids in membrane protein crystallization: A 31P-NMR study of colloidal properties of dihexanoyl phosphatidylcholine

✍ Scribed by Jean-Luc Eisele; Jean-Michel Neumann; Claude Chachaty

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 234 KB
• Volume: 55
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(90)90173-o

✦ Synopsis

The colloidal features of short chain phospholipids can be deduced from 3'P-NMR analysis by comparison with available data on phospholipid aqueous dispersion. In this study with dihexanoyl phosphatidylcholine, detergent phase separation was obtained by temperature shift and by addition of the precipitating agent polyethylene glycol. The 3'P-NMR spectra indicate that the detergent micelles fuse to enter the hexagonal Ha~ and lamellar phases. Consequences for the crystallization of membrane proteins are discussed.