Shoot regeneration of hybrid seed geranium (Pelargonium x hortorum) and regal geranium (Pelargonium x domesticum) from primary callus cultures
✍ Scribed by Kerry B. Dunbar; Christine T. Stephens
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 772 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0167-6857
No coin nor oath required. For personal study only.
✦ Synopsis
Procedures have been developed that increase the rate of shoot regeneration of hybrid seed geranium from month-old primary callus cultures. Hybrid geranium callus tissue covered with green nodular structures was initiated by placing shoot tip explants on solidified Murashige & Skoog medium (MS) supplemented with 2.0 mg 1-l zeatin and 1.9 mg 1-i indoleacetic acid. Hybrids Red Orbit, White Orbit and Scarlet Orbit were shown to produce 5-50 shoot primordia per explant when callus was initiated on this medium. Regal geranium callus was initiated by placing leaf explants on MS medium supplemented with 2.0 mg 1-l 6-benzylaminopurine and 2.0mg 1-1 naphthaleneacetic acid. Regal geranium cultivars Tiny Tot and Lavender Grand Slam were shown to produce between 2-50 shoot primordia per explant when initiated on the same medium.