Shoot regeneration from tissue-cultured leaves of the American cranberry (Vaccinium macrocarpon)
✍ Scribed by Michael Marcotrigiano; Susan P. McGlew; Grant Hackett; Bindu Chawla
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 714 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0167-6857
No coin nor oath required. For personal study only.
✦ Synopsis
A method for shoot regeneration from leaf explants in two cultivars of cranberry (Vaccinium macrocarpon Ait.) is described. Modified Anderson's medium supplemented with combinations of thidiazuron (TDZ) with or without 1 luM NAA (a-naphthaleneacetic acid) was used to optimize shoot regeneration. The effect of light or dark incubation was also determined. Maximum regeneration was obtained in the light in the presence of 10 ~M TDZ and 1 IJ-M NAA. While this medium was suitable for leaf explants obtained from shoot cultures, regeneration did not occur from leaves collected from greenhouse-grown plants. Elongation of the regenerated shtSot tips did not occur until explants were transferred to growth regulator-free medium at which time only a minority of shoots elongated. Elongated shoots could be dissected away from leaf tissue, rooted easily, and acclimitized to ambient conditions.