✦ LIBER ✦

Shedding of infectious virus and virus antigen during acute infection with respiratory syncytial virus

✍ Scribed by Matti Waris; Olli Meurman; Maurice A. Mufson; Olli Ruuskanen; Pekka Halonen

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 677 KB
Volume: 38
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890380208

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Shedding of respiratory syncytial virus (RSV) in nasopharyngeal aspirates (NPA) of hospitalized children with acute respiratory infection was studied using direct antigen detection by time‐resolved fluoroimmunoassay, rapid identification of infectious virus in centrifugally inoculated cell cultures by immunoperoxidase staining and conventional virus culture. Sequential NPAs, in which also local RSV‐specific IgA response was measured, were collected from children with proven RSV infection. The shedding pattern was similar for both infectious virus and viral antigen. The overall agreement of the three methods was good (81%) in diagnostic specimens collected on admission, but markedly reduced (46%) in follow‐up specimens. Secretory IgA was abundant in specimens giving discrepant or negative results only. The proportion of patients who shed RSV was high (⩾87%) in the first week after on‐set of symptoms, and decreased sharply in the second week. An opposite temporal pattern was found in the proportion of patients with detectable RSV‐IgA in their secretions. Sequentially isolated strains were antigenically stable as determined by their reactivity with a large panel of monoclonal antibodies. The findings suggest that RSV shedding should be monitored by using more than one method for virus detection. © 1992 Wiley‐Liss, Inc.

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