Sex pheromones of the yeastHansenula wingeiand their relationship to sex pheromones inSaccharomyces cerevisiaeandSaccharomyces kluyveri

✍ Scribed by Naohiko Yanagishima; Hiroaki Fujimura

• Publisher: Springer
• Year: 1981
• Tongue: English
• Weight: 379 KB
• Volume: 129
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00414698

✦ Synopsis

The yeast, Hansenula wingei has two mating types designated 5 and 21. Cells of each mating type were found to produce mating type-specific sex pheromone which induces sexual agglutinability of the opposite mating type. Crude fractions of these pheromones were prepared by using an Amberlite C G 5 0 (H + type) column. The agglutinabilityinducing action of the pheromones required glucose as carbon source, but no external nitrogen source. The action of the pheromones was inhibited by 5 gg/ml cycloheximide. The optimum pH for the pheromone action was 4.0. c~ Pheromones of Saccharomyces eerevisiae and Saccharomyces kluyveri induced sexual agglutinability of 5 mating type cells but did not that of 21 mating type cells, a Pheromones of the Saccharomyces yeasts had no effect on both 5 and 21 mating type cells. The sex pheromones of H. wingei had no effect on the sexual agglutinability of inducible a cells of S. cerevisiae. F r o m the experimental results obtained so far, we propose to call 5 and 21 mating types in H. wingei a and c~ mating types, respectively.