Sex pheromone blend of Manduca sexta: Responses of central olfactory interneurons to antennal stimulation in male moths
✍ Scribed by Dr. Thomas A. Christensen; John G. Hildebrand; James H. Tumlinson; Robert E. Doolittle
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 758 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0739-4462
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✦ Synopsis
Recently, chemical analysis of solvent rinses of t h e external surfaces of phero m o n e glands from female Manduca sexta revealed a blend of 12 aldehydes, including t h e previously identified sex pheromone component, (€,Z)-10,12hexadecadienal (bombykal). Previous electrophysiological studies showed that olfactory (deutocerebral) interneurons in t h e antennal lobes of males exhibited a wide range of responsiveness to pheromonal stimulation of t h e ipsilat-era1 antenna. These experiments were performed with c r u d e extracts of pheromone glands as well as two synthetic compounds: t h e major pherom o n e component, bombykal, and (€,Z)-ll,13-pentadecadienal, a mimic of a second component of t h e female's pheromone blend. Using intracellular methods, we have now reexamined similar olfactory interneurons, using each of t h e 12 chemically identified components as well as synthetic blends of vario u s combinations of them. Eight of t h e 12 components isolated from female glands elicited s o m e form of response in olfactory interneurons in males. In accordance with biochemical and behavioral data, the most potent are bombykal and two trienals, (€,€,€)-and (f,€,Z)-10,12,14-hexadecatrienal. We also conclude that t h e C75 dienal is selective for o n e of t h e trienal receptors o n t h e antenna, but is much less potent than t h e natural trienal stimulant.