Sex-linked control of sex pheromone behavioral responses in European corn-borer moths (Ostrinia nubilalis) confirmed with TPI marker gene
✍ Scribed by Thomas Glover; Marlene Campbell; Paul Robbins; Wendell Roelofs
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 732 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0739-4462
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✦ Synopsis
Abstract
In two races of European corn‐borer moths (ECB), the E‐race females emit and males respond to 99:1 sex pheromone blend of (E)/(Z)‐11‐tetradecenyl acetates, whereas the Z‐race females and males produce and respond to the opposite 3:97 pheromone blend of (E)/(Z)‐11‐tetradecenyl acetates, respectively. We previously have shown that female production of the final blend ratio is under control of a major autosomal locus but that the sequence of male upwind flight responses to the blend is controlled by a sex‐linked (Z‐linked) locus. This sex‐linked control of behavioral responses in crosses of E and Z ECB now is confirmed by use of sex‐linked TPI (triose phosphate isomerase) allozyme phenotypes to determine the origin of the sex chromosomes in F~2~ populations.
F~1~ males from reciprocal E × Z crosses generate similar behavioral‐response profiles in wind‐tunnel studies, with moderate numbers responding to the Z pheromone and intermediate blends (35%–65% Z), but very few responding to the E pheromone. The F~2~ behavioral‐response profiles indicate that they are composed of 1:1 mixtures of hybrids and paternal profiles. Analysis of TPI allozyme differences allowed us to separate male F~2~ populations into individuals whose Z chromosomes both originated from their grandfathers, and individuals who had one Z chromosome originating from each grandparent. With these partitioned F~2~s, the TPI homozygotes exhibited behavioral‐response profiles very much like their grandfathers, whereas the TPI hybrids produced response profiles similar to their heterozygous F~1~ fathers. These results demonstrate incontrovertibly that the response to sex pheromone in male ECB is controlled by a sex‐linked gene that is tightly linked to the TPI locus and therefore is independent of the locus controlling pheromone blend production in females.