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Sex identification by polymerase chain reaction using X-Y homologous primer

✍ Scribed by Nakahori, Yutaka ;Hamano, Koichi ;Iwaya, Makoto ;Nakagome, Yasuo


Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
197 KB
Volume
39
Category
Article
ISSN
0148-7299

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✦ Synopsis


A method of sex identification using the polymerase chain reaction technique is described. Using a pair of nucleotide primers from an X-Y homologous region, both the X and the Y sequences can be amplified simultaneously, and more importantly, they result in fragments of different lengths. The success of the procedure is therefore monitored by the presence of a X-specific band while sex is identified by the presence or absence of a Y-specific band.


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