Sex determination of forensic samples by dual PCR amplification of an X-Y homologous gene

✍ Scribed by Atsushi Akane; Satoko Seki; Hiroshi Shiono; Hiroaki Nakamura; Masanori Hasegawa; Masato Kagawa; Kazuo Matsubara; Yutaka Nakahori; Shigeo Nagafuchi; Yasuo Nakagome

• Book ID: 103902180
• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 673 KB
• Volume: 52
• Category: Article
• ISSN: 0379-0738
• DOI: 10.1016/0379-0738(92)90102-3

✦ Synopsis

Sex determination by polymerase chain reaction (PCR) analysis of the X-Y homologous amelogenin gene is highly reliable since the detection of an X-specific amplified fragment validates the procedure. Previously, we reported that 250 ng of template DNA are required for sex determination by this method. We report here a refinement of the technique to include dual PCR. Dual PCR using two sets of primers results in the detection of X- and Y-specific amplified fragments from as little as 0.005 ng of template DNA. This is a powerful technique for the analysis of trace forensic samples and its application is discussed.