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Sex determination of ancient human skeletons using DNA

✍ Scribed by Stone, Anne C.; Milner, George R.; Pääbo, Svante; Stoneking, Mark

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
28 KB
Volume
99
Category
Article
ISSN
0002-9483

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✦ Synopsis

A method for determining the sex of human skeletons was developed using molecular genetic techniques. The amelogenin gene, found on the X and Y chromosomes, was examined using the polymerase chain reaction (PCR) and a nonradioactive dot blot procedure. DNA was analyzed from 20 modern individuals of known sex and 20 skeletons from a n archaeological site in central Illinois dating to A.D. 1300. An independent assessment of the sex of each skeleton was made according to standard osteological methods. The sex of 19 ancient and 20 modern individuals was accurately determined using this molecular genetic technique. Molecular sex determination will be especially useful for juvenile and framentary remains when it is difficult, -or impossible, to "establish an features.

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