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Setup of a fluorescence lifetime and spectral correlated acquisition system for two-photon microscopy

✍ Scribed by Spriet, Corentin; Trinel, Dave; Laffray, Sophie; Landry, Marc; Vandenbunder, Bernard; Heliot, Laurent; Barbillat, Jacques

Book ID
119964196
Publisher
American Institute of Physics
Year
2006
Tongue
English
Weight
813 KB
Volume
77
Category
Article
ISSN
0034-6748

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## Abstract Two‐photon excitation fluorescence resonance energy transfer (2P‐FRET) imaging microscopy can provide details of specific protein molecule interactions inside living cells. Fluorophore molecules used for 2P‐FRET imaging have characteristic absorption and emission spectra that introduce