Sesquiterpenoids from a Cell Suspension Culture of the Liverwort Porella vernicosa Lindb
โ Scribed by Kanji Ono; Tomoko Sakamoto; Hironao Tanaka; Yoshinori Asakawa
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 223 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0882-5734
No coin nor oath required. For personal study only.
โฆ Synopsis
The liverwort Porella vernicosa Lindb. was cultured in vitro in Murashige and Skoog's medium. The pungent sesquiterpene dialdehyde, polygodial, was found as the major metabolite (20.3%). The second major metabolite was norpinguisone methyl ester (18.7%). In addition B -bisabolene (12.7%), drimenol (8.4%), cyclocolorenone (7.5%) and other drimane and pinguisane sesquiterpenoids were identified by GC-MS analysis. In the fieldgrown liverwort, the major metabolite was norpinguisone (24.5%) accompanied by polygodial (21.7%) and cyclocolorenone (13.4%). Thus the content of polygodial in the mixture of metabolites produced by P. vernicosa cultured in vitro was similar to that in the field-grown plant.
๐ SIMILAR VOLUMES
We demonstrate that adult human bone marrow (BM) contains a population of mesenchymal stromal cells (MSCs) that can be expanded in non-adherent, cytokine-dependent, suspension culture conditions for at least 42 days. The cells generated during suspension culture lacked detectable levels of gene expr