Serum type III procollagen and basement membrane proteins as noninvasive markers of hepatic pathology in Indian childhood cirrhosis
✍ Scribed by Premila Trivedi; Juha Risteli; Leila Risteli; M. Stuart Tanner; Sheila Bhave; Anand N. Pandit; Alex P. Mowat
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 586 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0270-9139
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✦ Synopsis
While serum concentrations of antigens of the aminopropeptide of type 1 1 1 procollagen have been considered as indicators of hepatic pathology in adults, the high concentrations normally found in children during growth may preclude their use in pediatric liver disease.
To clarify this and to determine the role of other circulating c o n n d v e t h e -r e l a t e d substances in children, we have measured serum concentrations of antigens related to aminopropeptide of type III procollagen, the 7s domain of type IV collagen and the P1 fragment of leFninin in healthy subjects aged 1 month to 4 yeers and in children with Indian childhood cirrhosis, a particularly aggressive form of liver disease. In healthy subjects, there was a considerable age variation in serum aminopropeptide of type III procollagen but not in 75 collagen or laminin P1. In Indian childhood cirrhosis, all three serum antigens were increased (p < 0.001) above the upper limit of normal for age. Both the serum 75 collagen and laminin P1 concentrations showed a signifhnt correlation with the degree of intralobular fibroscis and also with the severity of necrosis and cellular infiltration, suggesting that these serum antigens may be a noninvasive means of asdlessing and monitoring events aeeociated with hepatic fibrosis in Indian childhood cirrhosis. The raised serum aminopropeptide of type III procollagen in Indian childhood cirrhosis did not correlate with any histological parameter 888888ed. Gel filtration of serum showed that, in healthy subjects, the predominant antigenic form of aminopropeptide of type 1 1 1 procollagen was a degradation peptide smaller than authentic aminopropeptide of type 1 1 1 procollagen; while in Indian childhood cirrhosis the authentic peptide and a larger degradation peptide predominated. These findings suggest that, in Indian childhood cirrhosis, serum aminopropeptide of type 111 procollagen raised above the normally high levels found in children reflect,