Serum glutamic-oxalacetic-transaminase activity as an index of liver-cell injury from cancer. A preliminary report
✍ Scribed by Felix Wroblewski; John S. Ladue
- Publisher
- John Wiley and Sons
- Year
- 1955
- Tongue
- English
- Weight
- 640 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
LUTAMIC:-OXAI ACETIC transaminase is wide-G ly distributed in animal tissues. Its greatest concentration, however, is in heart muscle, skeIetaI muscle, brain, liver, and kidney in decreasing order.1 We have already shown that this enzyme when measured in seruni is elcvated after acutc myocardial infarction.2 This finding led us to study its concentration in liver diseases, since the enzyme is present in relatively high concentration in liver.
Glutamic-oxalacetic tramaminaye is present in all human sera and will henceforth be referred to as SGO-T. Comparable concentrations are found whether chromatographic or spectrophotometric methods are eniployed.3 When serum is added to excesses of aspartic acid arid a-ketoglutaric acid buffered at optinial PH in the presence of coenzyme 1 (DPNH~) and malic dehydrogenase, SGO-T can lie measured in a spectrophotometer by the decrease in optical density resulting from the oxidation of DPNH? to DPN (Fig. 1). One unit of SGO-T activity represents a change in optical density of 0.001 per ml. per minute at wave lengths of 340 rnp. All tests done in this series of patients were performed with 0.5 nil. or 0.2 ml. of serum at room temperatures (24" to 30" C.). (The micromolar extinction coefficient of the D P N H ~ is 2.05.)
T h e studies made after myocardial infarction indicate that the enzyme is liberated into the blood stream following injury to the ceL4 [f this also proved to be the case in liver disease, the SGO-T level therefore ought to provide an index of Iiver-cell destruction if the enzyme is released from damaged liver cells. The level and persistence of increased activity