𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Serum-dependent regulation of α-aminoisobutyric acid uptake in bovine granulosa cells

✍ Scribed by W. Ross Allen; Marit Nilsen-Hamilton; Richard T. Hamilton; Denis Gospodarowicz

Book ID
102883219
Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
887 KB
Volume
98
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

The removal of serum from the medium of ovarian granulosa cells in exponential or confluent stages of growth results in a rapid and pronounced decrease in the rate of transport of the non‐metabolizable amino acid, α‐aminoisobutyric acid. This decrease is rapidly and completely reversed by the addition of serum. The decrease and its reversal are insensitive to inhibitors of RNA and protein synthesis and are unaffected by a number of other metabolic inhibitors. The serum requirement cannot be replaced by peptide hormones known to stimulate cell division and secretion by these cells. These data are consistent with a model of post‐translational control of AIB transport by a high‐molecular‐weight component of serum.

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## Abstract In the clonal PC12 pheochromocytoma cell line, the observed effects of nerve growth factor (NGF) on the uptake rates of α‐aminoisobutyric acid (AIB) depend upon the assay conditions employed. In orthodox uptake assays calling for serum removal prior to the addition of AIB, 50 ng/ml NGF

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✍ Dean H. Kedes; Peter W. Gunning; Eric M. Shooter 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 550 KB

## Abstract Nerve growth factor (NGF) stimulates the uptake rate of the nonmetabolized amino acid α‐aminoisobutyric acid (AIB) in the clonal PC12 pheochromocytoma cell line by 40–70%. This effect reaches a maximum after a 1‐hour incubation with the hormone and then drops over 50%, reaching a minimu