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Serum carboxypeptidase B: A spectrophotometric assay using protamine as substrate

✍ Scribed by Neal C. Corbin; Tony E. Hugli; Hans J. Müller-Eberhard

Book ID
102983359
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
608 KB
Volume
73
Category
Article
ISSN
0003-2697

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## N-Acetyl-L-phenylalanyl-L-34hiaphenylalanine has been shown to be a substrate for carboxypeptidase A. Hydrolysis of the compound obeys Michaelis-Menten kinetics with a KM of 0.22 mM and a k,,, of 6720 min-' at 22°C. A calorimetric assay, employing Ellman's reagent to detect the thiophenol relea