Serum antioxidant capacity in healthy and diabetic subjects as determined by enhanced chemiluminescence
✍ Scribed by Pinzani, Pamela ;Petruzzi, Enrico ;Orlando, Claudio ;Gallai, Roberta ;Serio, Mario ;Pazzagli, Mario
- Publisher
- John Wiley and Sons
- Year
- 1998
- Weight
- 64 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0884-3996
No coin nor oath required. For personal study only.
✦ Synopsis
Free radicals are considered to be important factors involved in many physiopathological processes. Several methods have been proposed for studying the mechanisms of antioxidant protection against free radical-induced injury, including the measurement of the total antioxidant capacity (TAC) in body ¯uids, based on enhanced chemiluminescence. This technique is calibrated against Trolox 2 and assay results are expressed as mmol/L of Trolox equivalents. Since many of the complications induced by diabetes appear to be mediated by oxygen free radical generation, we have investigated serum antioxidant capacity in a group of healthy subjects and in insulin-dependent diabetic (IDDM) subjects. A statistically signi®cant difference was noticed in TAC values between the IDDM group and the young control group. Even if the biological meaning of this signi®cant reduction in TAC remains to be explained, an overproduction of precursors of reactive oxygen free radicals and/or a decreased scavenger systems ef®ciency can be associated with the increased risk of atherosclerotic cardiovascular disease in diabetic patients.