Sertoli Cells: Methods and Protocols

✍ Scribed by Marco G. Alves,Pedro F. Oliveira (eds.)

• Publisher: Humana Press
• Year: 2018
• Tongue: English
• Leaves: 302
• Series: Methods in Molecular Biology 1748
• Edition: 1
• Category: Library

✦ Synopsis

This detailed volume explores the use of Sertoli cells as models in male reproductive biology or as supporters for other cell types, and it answers the need for standardized protocols for their in vitro culture. Prepared for non-specialists as well as experienced researchers that may be interested in a multidisciplinary approach to study these cells, the book examines these remarkable cells that present some unique features that go far beyond the physical and nutritional support of spermatogenesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Sertoli Cells: Methods and Protocols serves as a valuable resource for experienced researchers in reproductive biology, particularly andrologists, but also for future investigators and young students starting to study these extraordinary cells.

✦ Table of Contents

Front Matter ....Pages i-xiii
Establishment of Primary Culture of Sertoli Cells (Raquel L. Bernardino, Marco G. Alves, Pedro F. Oliveira)....Pages 1-8
Evaluation of the Purity of Sertoli Cell Primary Cultures (Raquel L. Bernardino, Marco G. Alves, Pedro F. Oliveira)....Pages 9-15
Preparation of Testicular Samples for Histology and Immunohistochemistry (Barbara Bilinska, Anna Hejmej, Malgorzata Kotula-Balak)....Pages 17-36
Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age (Valeria Grieco, Barbara Banco)....Pages 37-47
Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy (Jesús Martínez-Hernández, Vicente Seco-Rovira, Ester Beltrán-Frutos, Victor Quesada-Cubo, Concepción Ferrer, Luis Miguel Pastor)....Pages 49-60
Sertoli Cell Preparation for Co-immunoprecipitation (Maria João Freitas, Margarida Fardilha)....Pages 61-71
Profiling Signaling Proteins in Sertoli Cells by Co-immunoprecipitation (Maria João Freitas, Margarida Fardilha)....Pages 73-84
Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscopy (Marina G. Yefimova, Nadia Messaddeq, Annie-Claire Meunier, Anne Cantereau, Bernard Jegou, Nicolas Bourmeyster)....Pages 85-101
A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells (Nabil Eid, Yuko Ito, Akio Horibe, Hitomi Hamaoka, Yoichi Kondo)....Pages 103-112
Assessing Autophagy in Sertoli Cells (Chao Liu, Jehangir Khan, Wei Li)....Pages 113-128
Molecular Mechanisms and Signaling Pathways Involved in the Nutritional Support of Spermatogenesis by Sertoli Cells (Luís Crisóstomo, Marco G. Alves, Agostina Gorga, Mário Sousa, María F. Riera, María N. Galardo et al.)....Pages 129-155
Assessing Sertoli Cell Metabolic Activity (Ivana Jarak, Pedro F. Oliveira, Gustavo Rindone, Rui A. Carvalho, María N. Galardo, María F. Riera et al.)....Pages 157-171
Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy (Rita Ferreira, Fábio Trindade, Rui Vitorino)....Pages 173-190
Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs (Hong Wang, Qingqing Yuan, Minghui Niu, Liping Wen, Hongyong Fu, Fan Zhou et al.)....Pages 191-202
Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice (Diane Rebourcet, Annalucia Darbey, Michael Curley, Peter O’Shaughnessy, Lee B. Smith)....Pages 203-228
Regulation of Blood-Testis Barrier (BTB) Dynamics, Role of Actin-, and Microtubule-Based Cytoskeletons (Qing Wen, Elizabeth I. Tang, Nan Li, Dolores D. Mruk, Will M. Lee, Bruno Silvestrini et al.)....Pages 229-243
Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay (Haiqi Chen, Wing-yee Lui, Dolores D. Mruk, Xiang Xiao, Renshan Ge, Qingquan Lian et al.)....Pages 245-252
Computational Methods Involved in Evaluating the Toxicity of the Reproductive Toxicants in Sertoli Cell (Pranitha Jenardhanan, Manivel Panneerselvam, Premendu P. Mathur)....Pages 253-277
A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells (Anna Maggio, Raquel L. Bernardino, Patrizia Gena, Marco G. Alves, Pedro F. Oliveira, Giuseppe Calamita)....Pages 279-286
Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing (Yoni Baert, Jaime Onofre, Dorien Van Saen, Ellen Goossens)....Pages 287-294
Back Matter ....Pages 295-297

✦ Subjects

Cell Biology