Serotonin uptake and release from rat cerebellum in vitro

✍ Scribed by C. Beas-Zarate; M. E. Sandoval; A. Feria-Velasco

• Book ID: 102909468
• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 526 KB
• Volume: 12
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490120112

✦ Synopsis

Serotonin (5-HT) uptake and release were analyzed in cerebellar fractions. Cerebellar Pz fractions and molecular layer homogenates are able to accumulate exogenous 5-HT through an Na-dependent, high-affinity transport system. Molecular layer 5-HT uptake, however, was totally dependent upon Na+, whereas the accumulation of the arnine by P2 fractions from whole cerebellum was also carried out by an Na-independent transport system. Ca + +-dependent release of recently accumulated 5-HT was observed in both P2 fractions and molecular layer homogenates when depolarizing conditions were used. This release was blocked by ruthenium red and M g t f , two inhibitors of the Caf + entry to the nerve endings induced by depolarization. The molecular layer hornogenates released twice as much 5-HT as did cerebellar P2 fractions, indicating that serotoninergic boutons in the cerebellar cortex might be located in the molecular layer. Our results suggest that 5-HT might play a transmitter role in the cerebellum.