✍ Scribed by Karola Framberg; Hubert Mayer; Jürgen Weckesser; Gerhart Drews
No coin nor oath required. For personal study only.
Serological investigations carried out with isolated O-antigens (lipopolysaccharides) of 12 strains of l~hodopseudomonas palustris showed that these O-antigens can be arranged into 3 distinct serotypes. These are identical with the three ehemotypes previously established.
The passive hemagglutination test carried out with isolated lipopolysaeeharides of the strains 2/2 and K/1 of chemotype I, strains 8/1 and 1 e5 of ehemotype II and strains 11/1, 15, 1 a 1 and 42 of chemotype III and their respective rabbit antisera showed that crossreactions can only be observed in between the ehemotypes. No cross-reactions were observed between O-antigens of different chemotypes. This result was essentially confirmed by gel-precipitation studies (according to Ouehter-Iony) and in immunoeleetrophoresis using the alkali-treated lipopolysaeeharides of strains 8/1 (chemotype II) and 11/1 (chemotype III) as test antigens and antisera against strains cf chemotypes I--III. Cross absorptions carried out with strains 11/1,15 and 1 a 1, all belonging to chemotype III, showed identity of their O-antigens.
The earlier established rather far-reaching parallelity of chemo-and serotypes existing in Enterobacteriaeeae is therefore also valid for the taxonomically remote Rhodospirillaceae. In contrast to Enterobacteriaceae the different serotypes of Rhodopseudomonas palustris arc not distinct taxa.
Additional studies carried out with isolated lipopolysaecharides of other species of Rhodospirillaeeae (Rhodopseudomonas capsulata, viridis and gelatinosa and Rhodospirillum rubrum) failed to show any cross reaetivities with the three serotypes of t~hodopseudomonas palustris.
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