Serological typing scheme for bk-like isolates of human polyomavirus

✍ Scribed by Dr. Wendy A. Knowles; Patricia E. Gibson; Sylvia D. Gardner

• Book ID: 102908024
• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 553 KB
• Volume: 28
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890280212

✦ Synopsis

Human polyomavirus BK-like isolates were subjected to restriction endonuclease analysis with the enzymes EcoRI and Hind III. End-point dilution was used to obtain homogeneous virus pools for DNA analysis and to remove JC virus from a mixed stock. The results of Hind III digestion suggested that two subgroups could be distinguished. Several BK-like isolates were purified and rabbit antisera raised. The isolates were compared with each other and with BK and JC viruses by haemagglutination-inhibition (HI) and by neutralisation. JC virus was serologically distinct, but all the other isolates showed some cross-reactivity. Two subgroups were again evident: GS and PG were with prototype BK in subgroup 1, and MG and IV were in subgroup 2. Two isolates, AS and SB, reacted with isolates of both subgroups 1 and 2 but were distinct from one another: their genome was similar to subgroup 1 isolates. Typing by HI or by neutralisation may form a basis for grouping BK-like polyomavirus isolates.