Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor

✍ Scribed by Miroslav Pohanka; Petr Skládal

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 240 KB
• Volume: 19
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200704003

✦ Synopsis

Abstract

Tularemia caused by the bacterium Francisella tularensis belongs to danger infective diseases, therefore timely diagnosis is an important part of protective activities. A novel approach for serological diagnostics of tularemia using a four‐channel amperometric immunosensor was proposed and evaluated on BALB/c mice as a model organism. The immunosensor was able to work with small volumes of serum samples (0.05 μL per assay) and diagnosed tularemic infection very early after immunization. From the 5^th^ day, it was possible to distinguish tularemic sera from control sera from mice immunized with Escherichia coli on the probability level of 0.99 (t‐test). Some 40 measurements per one hour can be realized with the developed procedure. The obtained results were confirmed by the standard indirect ELISA. The proposed immunosensor‐based approach seems promising for rapid detection of microbial pathogen infections.