Serodiagnosis of Leishmania donovani infections: assessment of enzyme-linked immunosorbent assays using recombinant L. donovani gene B protein (GBP) and a peptide sequence of L. donovani GBP
✍ Scribed by A.T.R. Jensen; S. Gasim; Torsten Moller; A. Ismail; A. Gaafar; M. Kemp; A.M. El Hassan; A. Kharazmi; T.M. Alce; D.F. Smith; T.G. Theander
- Book ID
- 104165124
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 516 KB
- Volume
- 93
- Category
- Article
- ISSN
- 0035-9203
No coin nor oath required. For personal study only.
✦ Synopsis
The repetitive sequence of Leishmania major gene B protein (GBP) has previously been shown to be a useful tool in the diagnosis of cutaneous leishmaniasis (CL). Here, we have assessed enzyme-linked immunosorbent assays (ELISAs) using recombinant L. donovani GBP (rGBP) and a peptide sequence of L. donovani GBP (GBPP) in the diagnosis of L. donovani infections in Sudan. The sensitivity of the rGBP ELISA in diagnosing visceral leishmaniasis (VL) and post kala-azar dermal leishmaniasis (PKDL) was 92% and 93%, respectively. In contrast, the sensitivity of the GBPP ELISA was 55% for VL and 63% for PKDL. Plasma antibody reactivity of donors with VL and PKDL remained high for an extended period after the end of treatment. Antibody-reactivity to rGBP and GBPP was detected in 7 1% and 14% of plasma samples from CL patients, respectively. Plasma from healthy Sudanese donors living in an area endemic for malaria but free of leishmaniasis was negative in both assays.