Serodiagnosis of Chlamydia pneumoniae infection using three inclusion membrane proteins
✍ Scribed by Chen Hongliang; Zhou Zhou; Hu Zhan; Zeng Yanhua; Li Zhongyu; Lin Yingbiao; Dai Guozhi; Wu Yimou
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 165 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0887-8013
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✦ Synopsis
Abstract
The Chlamydia pneumoniae genome‐encoded open reading frames Cpn0146, Cpn0147, and Cpn0308 were expressed as recombinant proteins for detecting C. pneumoniae‐specific antibodies in samples from three groups of individuals including 183 with C. pneumoniae‐associated respiratory infection (group I), 60 healthy blood donors (group II), and 32 with no known respiratory infection (group III). The recombinant Cpn0146 was recognized by 71 (38.8% positive recognition rate), 15 (25%) and 1 (3.1%), Cpn0147 by 75 (40.9%), 14 (23.3%), and 2 (6.3%), and Cpn0308 by 82 (44.8%), 16 (26.7%), and 0 (0%) samples from groups I, II, and III, respectively. The positive recognition rates with any of the three antigens were significantly higher in group I than those in groups II and III, suggesting that more individuals from group I were likely infected with C. pneumoniae. This conclusion was confirmed with a commercially available whole organism‐based ELISA kit (Savyon Diagnostics Ltd., Ashdod, Israel), which detected C. pneumoniae antibodies in 98 (64.1%), 26 (43.3%), and 4 (12.5%) samples from group I, II, and III, respectively. Comparing to the commercial kit, the recombinant antigen‐based detection assays displayed >97% of detection specificity and >87% of sensitivity, suggesting that these recombinant antigens can be considered alternative tools for aiding in serodiagnosis of C. pneumoniae infection. J. Clin. Lab. Anal. 24:55–61, 2010. © 2010 Wiley‐Liss, Inc.