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Serine/threonine protein phosphatases in Dictyostelium discoideum: No evidence for type I activity

✍ Scribed by Marie-Noëlle Simon; Thomas Winckler; Rupert Mutzel; Michel Véron; José Carlos da Costa Maia


Book ID
117054776
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
558 KB
Volume
184
Category
Article
ISSN
0006-291X

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Despite the physiological importance of serine/threonine protein phosphatase type 2B (PP2B/calcineurin), an accurate assay method of PP2B in crude tissue extracts has not been established. By using recombinant protein phosphatase inhibitor-1 as a substrate and ascorbic acid as an antioxidant, we dev