Development of an Assay Method for Activ
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Shinya Mitsuhashi; Hiroshi Shima; Kunimi Kikuchi; Keiichi Igarashi; Rei Hatsuse;
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Article
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2000
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Elsevier Science
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English
⚖ 96 KB
Despite the physiological importance of serine/threonine protein phosphatase type 2B (PP2B/calcineurin), an accurate assay method of PP2B in crude tissue extracts has not been established. By using recombinant protein phosphatase inhibitor-1 as a substrate and ascorbic acid as an antioxidant, we dev