Serine-derivatized gadonanotubes as magnetic nanoprobes for intracellular labeling
✍ Scribed by Amy A. Hassan; Bonita Tak-Yee Chan; Lesa A. Tran; Keith B. Hartman; Jeyarama S. Ananta; Yuri Mackeyev; Lingyun Hu; Robia G. Pautler; Lon J. Wilson; Adrian V. Lee
- Book ID
- 102104523
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 371 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1555-4309
- DOI
- 10.1002/cmmi.293
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✦ Synopsis
Abstract
Gadonanotubes (GNTs), which are powerful new T~1~‐weighted MRI contrast agents, were derivatized with serine amino acid substituents to produce water‐soluble (2 mg ml^−1^) ser‐gadonanotubes (ser‐GNs) as magnetic nanoprobes for intracellular labeling. The ser‐GNTs were used to efficiently label MCF‐7 human breast cancer cells (1.5 × 10^9^ Gd^3+^ ions/cell) with no observable cytotoxicity. Cell pellets derived from the ser‐GNT labeled cells give bright T~1~‐weighted MR images, confirming that the ser‐GNTs are a promising new nanoprobe technology for magnetic cell labeling and possibly for in vivo cellular trafficking. Copyright © 2010 John Wiley & Sons, Ltd.