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Serial analysis of gene expression: from gene discovery to target identification

โœ Scribed by Stephen L Madden; Clarence J Wang; Greg Landes


Book ID
114383285
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
508 KB
Volume
5
Category
Article
ISSN
1359-6446

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The use of serial analysis of gene expression (SAGE) to determine gene expression profiles is increasing because the technique can provide absolute transcript numbers in a digital format and identify new genes. We developed a miniSAGE technique, which uses only 1 microg total RNA and reduces the amo