Sequestration of connexin43 in the early endosomes: An early event of Leydig cell tumor progression

✍ Scribed by Dominique Segretain; Xavier Decrouy; Jim Dompierre; Denise Escalier; Nafis Rahman; Céline Fiorini; Baharia Mograbi; Jean-Pierre Siffroi; Ilpo Huhtaniemi; Patrick Fenichel; Georges Pointis

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 571 KB
• Volume: 38
• Category: Article
• ISSN: 0899-1987
• DOI: 10.1002/mc.10160

✦ Synopsis

Abstract

Connexins form gap junction channels that allow intercellular communication between neighboring cells. Compelling evidence has revealed that Cx are tumor‐suppressor genes and reduced Cx expression has been related with uncontrolled cell growth in tumors and transformed cells. In the present study, we addressed Cx transcriptional and posttranscriptional regulations during the earlier stage of testicular tumors confined to Leydig cells in a transgenic mice model. In situ hybridization indicated that connexin43 (Cx43) mRNA was highly expressed either at early tumorogenesis (3 m) characterized by intense proliferation of Leydig cells, or at advanced tumorogenesis (6–7 m) when tumor cells completely invaded the testis. In contrast, Cx43 protein analyzed by Western blotting or classic immunohistochemical analyses was present at the beginning of tumor progression, but was dramatically reduced as tumor advanced. Application of high‐resolution deconvolution microscopy to testis sections demonstrates that cells that proliferate exhibited an aberrant cytoplasmic Cx43 localization, in contrast to the expected plasma membrane Cx43 localization in normal Leydig cells. Dual immunofluorescence labeling with specific markers of cellular compartments shows that cytoplasmic Cx43 signal was mainly sequestered within early endosomes. Altogether, this study provides the first evidence that impaired Cx43 trafficking in endosomes is an early event associated with uncontrolled cell proliferation that could serve as a neoplastic marker. © 2003 Wiley‐Liss, Inc.