Sequential resonance assignment from two-dimensional inter- and intra-residue 15N–1H correlation spectra
✍ Scribed by Permi Perttu; Annila Arto
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 392 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0749-1581
- DOI
- 10.1002/mrc.821
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✦ Synopsis
Sequential assignment of amide resonances in proteins and peptides can conveniently be derived from twodimensional inter-residue 15 N i -1 H N .i-1/ and intra-residue 15 N i -1 H N i correlation spectra. The inter-residue 15 N i -1 H N .i-1/ correlation spectrum is generated by recording the 15 N i frequency evolution indirectly and subsequently transferring the magnetization to 1 H N .i-1/ of the preceding residue for direct detection. The flow of coherence is established by the (H)N(COCAHA)-TOCSY pulse sequence. Following the path from intra-residue correlation via inter-residue correlation to the next intra-residue correlation results in sequential assignment in two dimensions. This kind of assignment protocol is most amenable to re-establishing sequential assignments of target proteins perturbed by binding of ligands or alternatively signals of peptide ligands can be traced in studies of structure-function relationships by NMR. Copyright