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Sequential monitoring of peripheral blood lymphocyte subsets in rats

✍ Scribed by Sandy C. Thompson; Kerry M. Bowen; Prof. Robert C. Burton

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
882 KB
Volume
7
Category
Article
ISSN
0196-4763

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✦ Synopsis

The methodology of enumeration of T cell subsets in humans is well established and has widespread clinical application. Sequential monitoring of lymphocyte subsets in small animals requires a consistent and reliable methodology for staining and enumerating lymphocyte populations. The optimal conditions for enumerating subclasses of rat peripheral blood lymphocytes with the Spec-trum 111 flow cytometer are described. The sources of biological variation and technical error that were considered in optimizing the techniques and the limitations in applying them are discussed.

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Platelet fatty acids and peripheral blood lymphocyte subsets in an institutionalized elderly population
✍ Widjaja Lukito; Jonathan M. Hodgson; Paul E. Hutchinson; Mark L. Wahlqvist; Step πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 47 KB

The associations between platelet fatty acids and peripheral blood lymphocyte subsets were studied in 78 institutionalized elderly individuals (27 men and 51 women), aged 67 to 100. Platelet fatty acids were assessed by gas chromatography, and peripheral blood lymphocyte subsets were quantitated by