## ABSTRACT To estimate the glomerular filtration rate (GFR) sequentially in conscious rats, we validated a single‐blood‐sample method using a bolus injection of the nonionic contrast medium iodixanol as a tracer. First, to clarify basal age‐related GFR profiles, iodixanol was intravenously adminis
✦ LIBER ✦
Sequential measurements of glycolytic and tricarboxylic acid cycle metabolites in single sample aliquots
✍ Scribed by Charles Colson; Michael H. Klapper
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 442 KB
- Volume
- 97
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Standard, pyridine nucleotide-linked fluorometric assays have been sequenced so that 14 glycolytic and tricarboxylic acid cycle intermediates can be measured in only two sample aliquots. This multiple assay procedure, which is based on commercially available enzymes and reagents, allows analysis on small tissue samples in relatively short times.
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