Sequential injection determination of D-glucose by chemiluminescence using an open tubular immobilised enzyme reactor
✍ Scribed by Xuezhu Liu; Elo Harald Hansen
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 983 KB
- Volume
- 326
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
A sequential injection analysis system is described that incorporates a nylon tubular reactor containing immobilised glucose oxidase. allowing determination of o-glucose by means of subsequent luminol chemiluminescence detection of the hydrogen peroxide generated in the enzymatic reaction. The operating parameters were optimised by fractional factorial screening and response surface modelling. The linear range of o-glucose determination was 30-600 PM, with a detection limit of 15 pM using a photodiode detector. The sampling frequency was 54 hK'. Lower LOD (0.5 pM o-glucose) could be reached by using a PMT as the detector. Fermentation broth samples were determined and good recoveries were obtained.