𝔖 Bobbio Scriptorium
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Sequential hydration of a dry globular protein

✍ Scribed by P. L. Poole; J. L. Finney

Book ID
101719585
Publisher
Wiley (John Wiley & Sons)
Year
1983
Tongue
English
Weight
287 KB
Volume
22
Category
Article
ISSN
0006-3525

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✦ Synopsis

Using direct difference ir and laser Raman spectroscopy, the sequential hydration of hen egg-white lysozyme was monitored. The ir data allowed us to identify some specific molecular hydration events that occur as water is added, whereas the Raman is interpreted in terms of conformational changes. The largest of these solvent-induced changes occurs below the hydration level a t which activity commences.

πŸ“œ SIMILAR VOLUMES

Sequential hydration of dry proteins: A
Sequential hydration of dry proteins: A direct difference IR investigation of sequence homologs lysozyme and Ξ±-lactalbumin
✍ P. L. Poole; J. L. Finney πŸ“‚ Article πŸ“… 1984 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 870 KB

## Abstract Direct difference ir spectra are presented as a function of hydration for lysozyme and α‐lactalbumin, and detailed sequential hydration molecular events identified. Despite the strong sequence homology between the two proteins, and their expected conformational similarity, the hydration