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Sequential diffusion of ammonium and nitrate from soil extracts to a polytetrafluoroethylene trap for 15N determination

✍ Scribed by Peter Sørensen; Erik Steen Jensen


Book ID
102624522
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
277 KB
Volume
252
Category
Article
ISSN
0003-2670

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✦ Synopsis


A novel diffusion method was used for preparation of

NHfand NO;-N samples from soil extracts far 15N determination. Ammonium, and nitrate following reduction to ammonia, are allowed to diffuse to an acid-wetted glass filter enclosed in polytetrafluoroethylene tape. The method was evaluated with simulated soil extracts obtained using 50 ml of 2 M potassium chloride solution containing 130 pg of NH:-N (2.3 atom% I5 N) and 120 b$g of NOT-N (natural "N abundance). No cross-over in the "N abundances of NH:-N and Nor-N was observed, indicating a quantitative diffusion process (72 h, 25O C). Owing to the presence of inorganic nitrogen lSN enrichments should be corrected for rhe blank nitrogen content.