Sequential determination of sugar units in reducing disaccharides by capillary gas chromatography/Fourrier-transform infrared spectroscopy
✍ Scribed by das Neves, H. J. Chaves ;Riscado, A. M. V.
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 370 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0935-6304
No coin nor oath required. For personal study only.
✦ Synopsis
Conversion of disaccharide reducing units into the corresponding methoximes followed by their reduction with NaBH4CN to the corresponding deoxy(methoxyamino)alditol glycosides (RCH2-NHOCH3) introduces a constitutional asymmetry in the sugar molecule. After methylation of the resulting compounds, cleavage of the glycosidic bonds is carried out by BF3-catalyzed methanolysis, affording permethylated 0-methylglycosides and permethylated deoxy(methylmethoxyamino)alditols with a free -OH corresponding to the position of the glycosidic bond. Identification of the reducing moiety is achieved by GC-FTIR and computerized retrieval of library FTIR spectra.