Sequential dark–photo fermentation and autotrophic microalgal growth for high-yield and CO2-free biohydrogen production
✍ Scribed by Yung-Chung Lo; Chun-Yen Chen; Chi-Mei Lee; Jo-Shu Chang
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 755 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0360-3199
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✦ Synopsis
Dark fermentation, photo fermentation, and autotrophic microalgae cultivation were integrated to establish a high-yield and CO 2 -free biohydrogen production system by using different feedstock. Among the four carbon sources examined, sucrose was the most effective for the sequential dark (with Clostridium butyricum CGS5) and photo (with Rhodopseudomonas palutris WP3-5) fermentation process. The sequential darkephoto fermentation was stably operated for nearly 80 days, giving a maximum H 2 yield of 11.61 mol H 2 / mol sucrose and a H 2 production rate of 673.93 ml/h/l. The biogas produced from the sequential darkephoto fermentation (containing ca. 40.0% CO 2 ) was directly fed into a microalga culture (Chlorella vulgaris CeC) cultivated at 30 C under 60 mmol/m 2 /s illumination. The CO 2 produced from the fermentation processes was completely consumed during the autotrophic growth of C. vulgaris CeC, resulting in a microalgal biomass concentration of 1999 mg/l composed mainly of 48.0% protein, 23.0% carbohydrate and 12.3% lipid.