Sequential centrifugal layer chromatography (SCLC): A new technique for the isolation of natural compounds. Part 2: Comparative study on centrifugal layer chromatography (CLC) and SCLC for the separation of furocoumarin isomers

✍ Scribed by Erdelmeier, C. A. J. ;Nyiredy, Sz. ;Sticher, O.

• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 243 KB
• Volume: 8
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240080305

✦ Synopsis

The new sequential centrifugal layer chromatography extends the possibilities for preparative separation of furocoumarin isomers. In the present work, isobergapten, pimpinellin, bergapten, isopimpinellin, and sphondin were isolated from a prepurified extract of Heracleum sphondylium roots using two different techniques, namely, centrifugal layer chromatography (CLC) and sequential centrifugal layer chromatography (SCLC). The former technique was carried out with four plates, whereas the latter method required only one plate, using the same mobile phase in each case. A further advantage of the SCLC method was that all completely separated compounds could be quickly and efficiently eluted in small volumes of methanol.