Sequential actions of glucose oxidase and peroxidase in molecular films assembled by layer-by-layer alternate adsorption

✍ Scribed by Mitsuhiko Onda; Yuri Lvov; Katsuhiko Ariga; Toyoki Kunitake

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 473 KB
• Volume: 51
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(19960720)51:2<163::aid-bit5>3.0.co;2-h

✦ Synopsis

Molecular films of protein/polyion layers were assembled by means of alternate adsorption through electrostatic interaction. Glucose oxidase (GOD) and peroxidase (POD) were assembled in combination with sodium poly(styrenesulfonate) (PSS) and poly(ethy1eneimine) (PEI), respectively. Enzyme activities of those films on specific substrates (glucose and H202) were examined by coloring reaction of dye DA67. A multienzyme film containing GOD layer and POD layer was prepared by alternate adsorption of POD/PSS followed by PEVGOD. Sequential redox reaction of glucose/H,OJDA67 was demonstrated successfully with this supramolecular system. 01996 John Wiley & Sons, Inc.