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Sequencing of the ovine interleukin-8-encoding cDNA using the polymerase chain reaction

✍ Scribed by Isabelle Legastelois; Timothy Greenland; Philippe Arnaud; Jean-Francois Mornex; Geneviève Cordier


Book ID
116132487
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
362 KB
Volume
150
Category
Article
ISSN
0378-1119

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We have developed a polymerase chain reaction (PCR) method for sequencing of tobacco chloroplast genome. In a mixture containing chloroplast DNA, 5'-end-labeled oligonucleotide primer, Taq DNA polymerase and reaction buffer, we were able to sequence a segment of chloroplast 16S rRNA gene. The result