Sequencing of Short Peptides Using FAB Mass Spectrometry—Increased Information via Derivatization

Derivatization procedures to facilitate

Derivatization procedures to facilitate de novo sequencing of lysine-terminated tryptic peptides using postsource decay matrix-assisted laser desorption/ionization mass spectrometry

✍ T. Keough; M. P. Lacey; R. S. Youngquist 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 132 KB 👁 2 views

Guanidination of the e e-amino group of lysine-terminated tryptic peptides can be accomplished selectively in one step with O-methylisourea hydrogen sulfate. This reaction converts lysine residues into more basic homoarginine residues. It also protects the e e-amino groups against unwanted reaction