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Sequencing of Peptide Mixtures by Edman Degradation and Field-Desorption Mass Spectrometry

โœ Scribed by Yasutsugu SHIMONISHI; Yeong-Man HONG; Toyoko KITAGISHI; Takekiyo MATSUO; Hisashi MATSUDA; Itsuo KATAKUSE

Book ID
115119596
Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
856 KB
Volume
112
Category
Article
ISSN
1432-1327

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N-terminal sequence determination of pol
N-terminal sequence determination of polypeptides and peptide mixtures by Edman degradation combined with californium-252 plasma desorption mass spectrometry
โœ Per F. Nielsen; Bernhard Landis; Michal Svoboda; Klaus Schneider; Michael Przyby ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 765 KB

The combination of manual Edman degradation with 252Cf plasma desorption mass spectrometry has been developed as an efficient method of polypeptide sequence determination. Results obtained with a variety of peptides and small proteins demonstrate unequivocal, two-fold sequence data, at each step fro