Sequence/structure selective thermal and photochemical cleavage of yeast-tRNAPhe by UO3.0.CO;2-R/asset/equation/tex2gif-stack-1.gif?v=1&t=h5lm2802&s=dbd23f9474be8e91a8d456eddac5e2c49a5afe77" class="inlineGraphic">

✍ Scribed by Peter E. Nielsen; Niels Erik Møllegaard

• Book ID: 102659462
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 636 KB
• Volume: 9
• Category: Article
• ISSN: 0952-3499
• DOI: 10.1002/(sici)1099-1352(199605)9:3<228::aid-jmr243>3.0.co;2-r

✦ Synopsis

The uranyl(V1) ion, UO:+, cleaves yeast tRNAPhe both thermally and photochemically. Photochemical cleavage takes place at all positions but exhibits maxima at , C49 and As*. Furthermore, in the presence of stoichiometric concentrations of citrate, the cleavage is generally suppressed except that strong cleavage at positions G,, and Ca-Us0 persists, indicating the presence of a high-affinity metal-ion binding site. It is proposed that these photocleavage sites reflect the tertiary structure of the yeast tRNAme molecule in terms of D-loop/Tloop interaction and anticodon loop conformation and that uranyl-mediated photocleavage of RNA may be used as a probe of RNA tertiary structure, and in particular for identifying binding sites for divalent metal ions. Thus a high-affinity metal-ion binding site is inferred in the "central pocket" formed by the D-loop, the T-loop and the acceptor stem.