Sequences ofSaccharomyces cerevisiae 2 μm DNA improving plasmid partitioning inHansenula polymorpha
✍ Scribed by Bogdanova, Aliona I.; Kustikova, Olga S.; Agaphonov, Michael O.; Ter-Avanesyan, Michael D.
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 117 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0749-503X
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✦ Synopsis
Insertion of the HindIII-PstI fragment of Saccharomyces cerevisiae 2 microns DNA into the Hansenula polymorpha replicative plasmids decreases plasmid copy number and ensures their distribution to daughter cells at both mitotic and meiotic cell divisions. This suggests that the stabilization effect is caused by the improvement of plasmid partitioning. Deletion analysis revealed that the region of 2 microns DNA sequence responsible for the increase of mitotic stability of H. polymorpha plasmids involves the 2 microns STB locus and adjoining region. Further analysis demonstrated that the stabilization effect may depend on the number of 24-28 bp imperfect repeats which were found in several copies in the STB locus and adjoining region.